Journal: Genes & Diseases

Article Title: Blockade of co-inhibitory receptor immune checkpoint protein TIM3/CD366 augments the anti-cancer activity of CAR-T therapy in solid tumors: An ovarian cancer example

doi: 10.1016/j.gendis.2025.101978

Figure Lengend Snippet: Specific IFN-γ and TNF-α release of T lymphocytes transduced with TIM-3-silenced HER2-specific chimeric antigen receptor (CAR) or HER2-specific CAR. (A, B) TIM-3-silenced CAR-T cells and control T cells were co-incubated with Galectin-9 + or Galectin-9 – SKOV3 tumor cells (E:T ratio 5:1 or 10:1). At 20 h after coculture, a specific enzyme-linked immunosorbent assay was used to analyze the supernatant for IFN-γ cytokine-release. Results were presented as mean ± standard deviation. (C, D) The detection of TNF-α in the same culture supernatant. Results were presented as mean ± standard deviation. ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Human cervical cancer cell line HeLa, lentivirus packaging cell line HEK 293TD, and human ovarian cancer cell line SKOV3 were purchased from American Type Culture Collection (Manassas, Virginia, USA) and cultured in Dulbecco's modified Eagle's medium (Invitrogen, Grand Island, New York) supplemented with 10% heat-inactivated fetal bovine serum.

Techniques: Transduction, Control, Incubation, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Genes & Diseases

Article Title: Blockade of co-inhibitory receptor immune checkpoint protein TIM3/CD366 augments the anti-cancer activity of CAR-T therapy in solid tumors: An ovarian cancer example

doi: 10.1016/j.gendis.2025.101978

Figure Lengend Snippet: TIM-3 silencing augmented the anti-tumor activity of chimeric antigen receptor-T (CAR-T) cells in vivo . 2 × 10 6 SKOV3 tumor cells expressing luciferase were intraperitoneally inoculated in a xenograft mouse model, and 7 days after inoculation, the 2 × 10 6 HER2-specific CAR-T kdTim-3 cells or CAR-T cells, or untreated T cells were intraperitoneally administered. (A, B) Tumor growth was monitored using an in vivo imaging system. (C) Survival curve of 80-day post-treatment. ∗ P < 0.05 and ∗∗ P < 0.01.

Article Snippet: Human cervical cancer cell line HeLa, lentivirus packaging cell line HEK 293TD, and human ovarian cancer cell line SKOV3 were purchased from American Type Culture Collection (Manassas, Virginia, USA) and cultured in Dulbecco's modified Eagle's medium (Invitrogen, Grand Island, New York) supplemented with 10% heat-inactivated fetal bovine serum.

Techniques: Activity Assay, In Vivo, Expressing, Luciferase, In Vivo Imaging

Journal: NPJ Precision Oncology

Article Title: GLUD1 supports ovarian cancer progression by counteracting anoikis via ARAF/MEK/ERK signaling

doi: 10.1038/s41698-026-01349-6

Figure Lengend Snippet: A Volcano plot of GLUD1 expression in epithelial ovarian cancer (EOC) from GEO datasets. Colors indicate gene expression levels: red for high, blue for low, and gray for non-significant expression changes. B Venn diagram illustrating the overlap among DEGs, anoikis-related genes, and amino acid metabolism–related genes. C GLUD1 expression in the GSE27651 dataset. Gene expression levels are shown with red representing tumor tissues and blue representing normal controls. D Kaplan-Meier curves showing overall survival differences between high and low GLUD1 expression groups in EOC patients from the TCGA dataset. Red and blue lines represent high and low expression levels, respectively. E Western blot analysis of GLUD1 expression in OVCAR3 and SKOV3 cells cultured under detached conditions. GAPDH was used as a loading control. F Representative immunohistochemistry images (Upper) and quantification (Lower) of GLUD1 expression in para-tumor, primary, and metastatic EOC tissues. G Overall survival curve and disease-free survival curve for EOC patients with low or high expression of GLUD1 based on IHC staining index. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: The human embryonic kidney cell line HEK293T and human ovarian cancer cell lines SKOV3, OVCAR3, HO8910PM, ES-2, A2780, and Caov-3 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Gene Expression, Western Blot, Cell Culture, Control, Immunohistochemistry

Journal: NPJ Precision Oncology

Article Title: GLUD1 supports ovarian cancer progression by counteracting anoikis via ARAF/MEK/ERK signaling

doi: 10.1038/s41698-026-01349-6

Figure Lengend Snippet: A Western blot analysis of GLUD1 expression in ovarian cancer cells with GLUD1 overexpression or knockdown. B Representative fluorescence images of live (green) and dead (red) cells under detached conditions (left) and quantification of live dead cell ratios (right). C Flow cytometric analysis of apoptosis in ovarian cancer cells cultured under detached conditions and corresponding quantification. D Transwell migration assays of SKOV3 and OVCAR3 cells with GLUD1 overexpression or knockdown and quantification of migrated cells. E Wound healing assays assessing migratory capacity of SKOV3 and OVCAR3 cells and quantification of wound closure. All experiments were performed in three independent biological replicates. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: The human embryonic kidney cell line HEK293T and human ovarian cancer cell lines SKOV3, OVCAR3, HO8910PM, ES-2, A2780, and Caov-3 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Western Blot, Expressing, Over Expression, Knockdown, Fluorescence, Cell Culture, Migration

Journal: Journal of Translational Medicine

Article Title: Preclinical synergistic effects when combining a radiolabelled HER2-targeting single domain antibody with PARP inhibitor Olaparib

doi: 10.1186/s12967-025-07572-2

Figure Lengend Snippet: Flow cytometry evaluation of HER2 expression in DLD1, DLD1 BRCA2 −/− , and SKOV3 cells. Histogram plots showing HER2 expression (purple) in the cells. The control peak was obtained using an isotype control antibody (gray). (Error bars are depicted as SD)

Article Snippet: Human SKOV3 ovarian cancer cells (RRID: CVCL_0532, ATCC), human colorectal DLD-1 cancer cells (RRID: CVCL_0248, Horizon Discovery) and human colorectal DLD-1 BRCA2 −/− cancer cells (RRID: CVCL_HD57, Horizon Discovery) were cultured in RPMI1640 medium (Gibco).

Techniques: Flow Cytometry, Expressing, Control